Journal: iScience
Article Title: Probing subicular inputs to the medial prefrontal cortex
doi: 10.1016/j.isci.2021.102856
Figure Lengend Snippet: Functional connectivity between the dorsal subiculum and the prefrontal cortex (A) Overview of the experimental design. A viral vector containing ChR2 is injected into the dorsal subiculum (dSUB). Responses to light stimulation were recorded from cells in the dorsal anterior cingulate cortex (dACC), ventral ACC (vACC), prelimbic (PL) and infralimbic (IL) prefrontal cortex across different distances from bregma. (B) Image of the injection site at bregma −3.08 mm. Fibers can be seen in the retrosplenial cortex (RSP) and the medial mammillary nucleus (MM). Bi) Enlargement of the region in B. (C) Fibers in the prefrontal cortex at bregma +0.95 mm. Additionally, fibers are seen in the lateral septum (LSr) and the nucleus accumbens (NAc). Ci) Enlargement of the region in C showing fibers in the ACC, but not the IL. (D) Fibers in the prefrontal cortex at bregma +1.42 mm. Additionally, fibers can be seen in the nucleus accumbens and lateral septum. Di) Enlargement of the region in D showing fibers in the superficial layers of the PL and the deep layers of the IL. (E and F) A recorded neuron in the (E) vACC and the (F) IL on the left with the light-induced response on the right. Blue tick indicates the time point of LED stimulation. (B–D) Scale bar 500 μm. (E–F) Scale bar 100 μm. Green, eYFP; Blue, DAPI; Red, biocytin-streptavidin. All images are obtained from the same animal.
Article Snippet: Cells were recorded in the prefrontal cortex, spanning the infralimbic cortex (IL), prelimbic cortex (PL) and the dorsal and ventral parts of the anterior cingulate cortex (ACC) using the MultiClamp 700B Amplifier (Molecular Devices, California, USA).
Techniques: Functional Assay, Plasmid Preparation, Injection
